Figure 9.

The IGGH Domain Mediates Homodimerization in Planta.

Bimolecular fluorescence complementation analysis of the ability of IGGH domains to form homodimers. Fluorescence microscopy pictures, showing merged fluorescence signals from chloroplasts (red) and YFP (green) are shown to the left. A protoplast transformed with the two different YFP-IGGH constructs and an untransformed protoplast are shown in the top left picture. The bottom left picture shows a representative protoplast from one of the three negative controls. Bright-field microscopy images of the protoplasts are shown in the middle. The construct combination analyzing IGGH-mediated reconstitution of functional YFP (N-term YFP:IGGH + C-term YFP:IGGH) and the three negative controls (N-term YFP:IGGH + C-term YFP, N-term YFP + C-term YFP:IGGH, and N-term YFP + C-term YFP) are shown to the right.