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. 2010 Feb 9;22(2):364–375. doi: 10.1105/tpc.109.071209

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© 2010 American Society of Plant Biologists

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Figure 2. — PAS1 Interacts with Elongase Enzymes in the ER of Arabidopsis Epidermal Cells.

(A) Coexpression of 35S:GFP-PAS1 (left) and ER marker CD3-959:mCherry (middle) showed colocalization (merged, right).

(B) Coexpression of the split YFP pairs YFP^N-PAS1/KCR-YFP^C, YFP^N-PAS2/ YFP^C-PAS1, and YFP^N-CER10/KCR-YFP^C led to BiFC of YFP (green). Chloroplast autofluorescence is red.

(C) Coexpression of split YFP pairsYFP^N-PAS1/ YFP^C-DPL1, YFP^N-DPL1/ YFP^C-PAS1, and YFP^N-CER10/YFP^C-DPL1 did not produce any YFP fluorescence.

(D) Coexpression of YFP^N-CER10/YFP^C-PAS1 GFP (left) and ER marker CD3-959:mCherry (middle) showed BiFC colocalization in the ER (merged, right).

Bars = 10 μm in (A) to (C) and 40 μm in (D).