Figure 5.

BFA-Dependent PIN1-GFP Aggregation Is Enhanced in pas1 Mutant Cells.

(A) BFA induction of PIN1-GFP aggregation in pas1. In contrast with the wild type (left), PIN1-GFP aggregation can be observed in the pas1-3 mutant (right) even in the absence of BFA (0 μM) and is strongly enhanced with 25 μM BFA treatment.

(B) The pas1-3 mutation enhances sensitivity to BFA. The relative number of vascular cells showing BFA compartments as illustrated in (A) at 100 μM was monitored in the wild type and pas1 according to the BFA concentration. Data are the mean of three replicates of 25 roots ± se.