Figure 7.

Biochemical Characterization of the sir1/cnx5 Mutant.

Leaf extracts of Arabidopsis sir1/cnx5 mutants with a deficiency in step 2 of Moco biosynthesis were analyzed for their cPMP contents (A) and MPT/Moco contents (B), as well as for the activities of NR (C), SO (D), AO (E), and XDH (F). cPMP and MPT/Moco were measured as CompoundZ (n = 3) and FormA dephospho (n = 3), respectively. NR and SO activities are given as mean ± sd(n = 4). For AO and XDH, activities of one representative gel of four experiments are shown.