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. 2010 Feb 19;22(2):321–334. doi: 10.1105/tpc.109.072199

Figure 1.

Figure 1.

The AGO4 Group Proteins Bind 24-Nucleotide sRNAs Produced by the RdDM Pathway.

(A) Immunoblots demonstrate that antipeptide antibodies to AGO4, AGO6, and AGO9 are specific, and the proteins show decrease in some RdDM mutants.

(B) RNA stability is not affected in the RNA interference mutants in which protein levels are decreased. The vertical axis represents the ratio of the average C(t) value of each AGO mRNA to ACTIN2 levels. Error bars represent an se of the ratio.

(C) AGO4, AGO6, and AGO9 preferentially bind 24-nucleotide sRNAs. Each panel contains the sRNA size profile of the total population or IP data sets. FLAG AGO4 (454) depicts the FLAG AGO4 C data set.

(D) The AGO4 group proteins have a 5′ nucleotide bias toward adenosine. Each panel denotes the percentage of sRNAs with 5′ nucleotide identities. For the total population, AGO4 IP and AGO9 IP immunopurified with the antipeptide antibody, and the 5′ nucleotide composition for the 24-nucleotide size class is indicated below the appropriate bar. Rep, replicate.