Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Feb 19;22(2):321–334. doi: 10.1105/tpc.109.072199

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 American Society of Plant Biologists

Open Access articles can be viewed online without a subscription.

PMC Copyright notice

Figure 4. — Functional Divergence among AGO4, AGO6, and AGO9.

(A) Immunoblot analysis (αμFLAG antibody) shows a similar level of AGO4, AGO6, and AGO9 when expressed under the AGO4 promoter. AGO1 levels were used as a loading control.

(B) RNA gel blot analyses of PAGO4:FLAG AGO6 and PAGO4:FLAG AGO9 in the ago4-3 background (bkgd) show locus-specific complementation of AGO4-dependent sRNAs.

(C) Bisulfite analyses of AtSN1 and SIMPLEHAT2 indicate that asymmetric methylation complementation by AGO6 and AGO9 correlates with the observed sRNA accumulation at each locus (PAGO4:FLAG AGO9 partially complements, while PAGO4:FLAG AGO6 does not complement). For AtREP2, PAGO4:FLAG AGO9 fully complemented CpHpG and CpHpH methylation, while PAGO4:FLAG AGO6 had partial, but significant, complementation of CpHpH but not CpHpG. The different cytosine contexts are indicated above the top panel. The vertical axis represents the average percentage of methylated cytosines (per context). Error bars represent 95% confidence limits. The SIMPLEHAT2 locus contained only cytosines in a CpHpH context. Sample labels appear below the AtREP2 panel. H represents the nucleotides A, T, or C.