Table 3.

TuMV-GFP and TuMV-AS9-GFP Infection in Single and Multiple rdr Arabidopsis Mutants

Virus	Arabidopsis Genotype	Plants	Inoculated Rosette Leavesa	Noninoculated Rosette	Bolt Tissue	Cauline Leaves	Inflorescences 7 DAI 15 DAI %b
TuMV-GFP
	Col-0	14	14	14	14	14	14	14	100
	rdr1-1	14	14	14	14	14	14	14	100
	rdr2-1	14	14	14	14	14	14	14	100
	rdr6-15	14	14	14	14	14	14	14	100
	rdr1-1 rdr2-1	14	14	14	14	14	14	14	100
	rdr1-1 rdr6-15	14	14	14	14	14	14	14	100
	rdr2-1 rdr6-15	14	14	14	14	14	14	14	100
	rdr1-1 rdr2-1 rdr6-15	14	14	14	14	14	14	14	100
	dcl2-1 dcl3-1 dcl4-2	14	14	14	14	14	13	14	100
TuMV-AS9-GFP
	Col-0	14	0	0	0	0	0	0	0
	rdr1-1	14	14	14	14	14	0	2	0.45
	rdr2-1	14	14	0	0	0	0	0	0
	rdr6-15	14	14	14	14	14	0	0	0
	rdr1-1 rdr2-1	14	14	14	14	14	0	0	0
	rdr1-1 rdr6-15	14	14	14	14	14	12	14	51
	rdr2-1 rdr6-15	14	14	14	14	14	0	0	0
	rdr1-1 rdr2-1 rdr6-15	14	14	14	14	14	9	14	49
	dcl2-1 dcl3-1 dcl4-2	14	14	14	14	14	14	14	100

Number of plants showing local and systemic infection were scored by GFP fluorescence under UV illumination. A total of 14 plants were analyzed. Except for a set of inflorescence samples, systemic infection data were from plants at 15 DAI.

^aLocal infection at 7 DAI.

^bPercentage (average of 14 plants) of inflorescence clusters showing GFP at 15 DAI.