Figure 3.

Effect of PGC-1 overexpression and knockdown on mRNA expression of genes involved in lipid metabolism and mitochondrial biogenesis and function. Total RNA was extracted from C2C12 myotubes infected with GFP, PGC-1α, or PGC-1β adenoviruses (A) or C2C12 myotubes treated with specific PGC-1α or PGC-1β siRNA pools (B). Expression was assessed by quantitative RT-PCR and normalized to TBP levels. Values are means ± se of 1 experiment with 4 replicates and are expressed relative to GFP-infected cells (A) or scrambled siRNA (B). ru, relative units. *P < 0.05, **P < 0.01, ***P < 0.001 vs. respective controls.