Figure 4.

Effects of PGC-1 expression on AMPK and ACC protein expression and phosphorylation. Proteins were extracted from C2C12 myotubes at 72 h postinfection, resolved by SDS-PAGE, transferred to 0.45-μm nitrocellulose, and detected using Western blots. A) Representative Western blot for phosphorylated AMPK, total AMPK, phosphorylated ACC, and total ACC. B) Quantification of Western blots. Values are means ± se of 4 independent experiments (2 to 3 replicas each) and are expressed relative to GFP-infected cells. *P < 0.05.