Figure 5.

Effect of PGC-1 on lipid composition of C2C12 myotubes in serum-replete (left panels) and serum-starved states (right panels). Lipid content and composition was determined in 72-h postinfection C2C12 myotubes incubated in DMEM-H with 2% (serum replete) or 0.1% serum (serum starved) and either BSA, oleate (Ole), or palmitate (Pal) for 24 h. Lipids were extracted with chloroform-methanol (2:1) and separated by thin-layer chromatography. Lipid classes containing fatty acids were also quantitated by GC/MS (as detailed in Materials and Methods). Values are means ± se of ≥2 independent experiments, each with 2 to 4 replicates for all conditions, with the exception of oleate exposure in serum-starved cells (n=1, 4 replicates). Data are expressed as milligrams lipid per milligram protein. A) Total lipids. B) Triglycerides. C) Diglycerides. D) Phospholipids. E) Cholesterol esters. *P < 0.05; **P < 0.01.