Figure 2.

Transgenic (nanoparticle-dependent) NMP colocalizes with endogenous RDS. Frozen retinal sections from eyes collected at multiple ages (PI-2 to PI-30) were immunostained for NMP (mAB 3B6, green) and total RDS (RDS-CT, red) with a nuclear counterstain (DAPI, blue). Transferred RDS from eyes injected with nanoparticles at P5 (A) and P22 (B) is detected beginning at PI-2. Expression remains strong through the latest time point analyzed (PI-30) and colocalizes with native RDS. Expression is limited to the OSs or nascent OSs and is not detected in any other retinal cell types, subcellular compartments, or layers. n = 3–5 mice/group. INL, inner nuclear layer. Scale bars = 20 μm.