Figure 4.

MOP-NMP nanoparticle injection does not elicit an acute cytokine response. qRT-PCR for TNF-α and IL-6 was completed at PI-2 and PI-30 on cDNA prepared from whole eyes for animals injected at P5 or P22. No significant differences were observed between TNF-α (A) or IL-6 (B) levels in either injected sample groups (MOP-NMP nanoparticle, naked DNA, or saline treatment) or uninjected animals. Significant cytokine expression was detected in identically prepared positive control samples from the B. cereus endophthalmitis model.