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. 2010 Apr;24(4):1261–1270. doi: 10.1096/fj.09-147207

Figure 2.

Figure 2.

Analysis of nonpolar retinoids extracted from the livers of RetSat−/− and RetSat+/+ mice. RetSat−/− and RetSat+/+ mice were gavaged with retinyl palmitate. Two hours post-treatment, livers were dissected, and retinoids were saponified, organically extracted, and examined by normal-phase HPLC. Extractions were normalized to the amount of tissue used. Retinoids were identified on the basis of their online UV-visual absorbance spectra and by their elution profiles compared with authentic standards. Peaks identified were peaks 1 (13-cis-retinol), 2 (DROL), and 3 (9,13-di-cis-retinol); other retinoid peaks eluting outside the 12- to 16-min range were omitted for clarity. The RetSat−/− mouse chromatogram (dashed line) shows that DROL was not detected in RetSat−/− mice gavaged with retinyl palmitate. Inset shows the UV-visual spectrum of DROL isolated from livers of RetSat+/+ mice.