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Levels of nonpolar retinoids in tissues of RetSat−/− and RetSat+/+ mice. All-trans-retinol (atROL) and retinyl esters (RE) extracted from liver, serum and visceral adipose tissues of RetSat−/− and RetSat+/+ mice were examined by normal-phase HPLC. Amounts loaded were normalized on the basis of the amount of tissue. Levels of retinoids were quantified on the basis of their absorbance over time (AUC) compared to that of known amounts of standards.
