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. Author manuscript; available in PMC: 2010 Mar 25.
Published in final edited form as: J Am Chem Soc. 2009 Jul 8;131(26):9361–9367. doi: 10.1021/ja9023836

Figure 3.

Figure 3

(a) SE-HPLC chromatograms of IgGDgn and IgGDNP complexes formed upon binding to multivalent ligands. Each antibody-ligand complex is labeled by the chromatogram. The schematic structures show the aggregates expected to be formed from IgG; other proteins with the same MW also seem to be present in small quantities. The products in a mixture of bis-DNP ligand 1 and IgGDNP showed the cyclic antibody dimer (IgG)212 (MW = 300 kDa and trimer (IgG)313 (MW = 450 kDa). The product of a mixture of tris-DNP ligand 2 to IgGDNP, as expected from previous work, was a bicyclic trimer complex. Mixing bis-Dgn ligand 5 with IgGDgn yielded multiple peaks that corresponded to cyclic dimer, trimer and tetramer as well as monomeric antibody. 13 (b) Calibration of the size-exclusion column using proteins with known molecular masses.