Figure 6.

The bottom SE-HPLC trace is the IgG^Dgn purified using the described procedure in Figure 5. IgG^Dgn was present as a mixture of cyclic complexes (monomer, dimer, trimer, and tetramer), as we did not have a weaker-binding monomeric ligand to dissociate its complexes. The middle trace is of the IgG^DNP purified using our protocol and the top trace is after mixing purified IgG^DNP with trisDNP (ligand 2).