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. Author manuscript; available in PMC: 2010 Mar 25.
Published in final edited form as: Biochem Pharmacol. 2008 May 21;76(3):426–436. doi: 10.1016/j.bcp.2008.05.012

Figure 2.

Figure 2

LDH Activity in Rat Pecision-Cut Liver Slices. PCLS were cultured at t0, 24, 48, 72 and 96 hours in the absence of ethanol (Control-open circles), presence of 25 mM ethanol (Ethanol-closed triangles), absence of 25 mM ethanol but in the presence of 0.50 mM 4-MP (Control + 4-MP-closed circles), and in the presence of both 25 mM ethanol and 0.50 mM 4-MP (Ethanol + 4-MP-closed squares). LDH activity (% Cytotoxicity) was determined as described in the Materials and Methods. Data are expressed as the mean % Cytotoxicity ± S.E.M. of five separate experiments. Ethanol significantly increased the % Cytotoxicity when compared to other 3 groups, *P < 0.05. Ethanol and Control PCLS groups compared to Ethanol + 4-MP or Control + 4-MP PCLS. Additionally, the Control PCLS showed significantly higher % Cytotoxicity compared to the Ethanol + 4-MP or Control + 4-MP PCLS, #P < 0.05.