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. Author manuscript; available in PMC: 2010 Mar 25.

Published in final edited form as: Biochem Pharmacol. 2008 May 21;76(3):426–436. doi: 10.1016/j.bcp.2008.05.012

Figure 5A. CYP2E1 Expression in Microsomes from Rat Precision-Cut Liver Slices. PCLS were cultured at t₀, 24, 48, 72 and 96 hours in the absence of ethanol (Control), absence of ethanol but the addition of 0.50 mM 4-MP (Control + 4-MP), presence of 25 mM ethanol (Ethanol), and in the presence of both 25 mM ethanol and 0.50 mM 4-MP (Ethanol + 4-MP). Microsomes were prepared as outlined in the Materials and Methods, loaded on SDS-PAGE gels (5 μg protein per lane), and analyzed by immunoblotting and densitometry as described in the Materials and Methods. The data are expressed as the mean ± S.E.M. of five separate experiments. Significantly decreased as compared to t₀, #P < 0.001. Significantly increased as compared to the corresponding control PCLS, *P < 0.05.

Figure 5B. CYP2E1 Activity in Microsomes from Rat Precision-Cut Liver Slices. PCLS were cultured at t₀, 24, 48, 72 and 96 hours in the absence of ethanol (Control), absence of ethanol but the addition of 0.50 mM 4-MP (Control + 4-MP), presence of 25 mM ethanol (Ethanol), and in the presence of both 25 mM ethanol and 0.50 mM 4-MP (Ethanol + 4-MP). Microsomes were assayed for the presence of CYP2E1 using p-Nitrophenol oxidation as described in the Materials and Methods. The data are expressed as the mean ± S.E.M. of five separate experiments. Significantly decreased as compared to t₀, #P < 0.001. Significantly increased as compared to the corresponding control PCLS, *P < 0.05.

Figure 5A. CYP2E1 Expression in Microsomes from Rat Precision-Cut Liver Slices. PCLS were cultured at t₀, 24, 48, 72 and 96 hours in the absence of ethanol (Control), absence of ethanol but the addition of 0.50 mM 4-MP (Control + 4-MP), presence of 25 mM ethanol (Ethanol), and in the presence of both 25 mM ethanol and 0.50 mM 4-MP (Ethanol + 4-MP). Microsomes were prepared as outlined in the Materials and Methods, loaded on SDS-PAGE gels (5 μg protein per lane), and analyzed by immunoblotting and densitometry as described in the Materials and Methods. The data are expressed as the mean ± S.E.M. of five separate experiments. Significantly decreased as compared to t₀, #P < 0.001. Significantly increased as compared to the corresponding control PCLS, *P < 0.05.

Figure 5B. CYP2E1 Activity in Microsomes from Rat Precision-Cut Liver Slices. PCLS were cultured at t₀, 24, 48, 72 and 96 hours in the absence of ethanol (Control), absence of ethanol but the addition of 0.50 mM 4-MP (Control + 4-MP), presence of 25 mM ethanol (Ethanol), and in the presence of both 25 mM ethanol and 0.50 mM 4-MP (Ethanol + 4-MP). Microsomes were assayed for the presence of CYP2E1 using p-Nitrophenol oxidation as described in the Materials and Methods. The data are expressed as the mean ± S.E.M. of five separate experiments. Significantly decreased as compared to t₀, #P < 0.001. Significantly increased as compared to the corresponding control PCLS, *P < 0.05.