Fig. 4.
The DHR38 mutation has minor effects on ecdysone-regulated gene expression at the onset of metamorphosis. RNA was isolated from DHR38Y214/CyO and Df(2)KetelRX32/CyO heterozygotes (control) or DHR38Y214/Df(2)KetelRX32 mutants (DHR38−) and analyzed by northern blot hybridization. RNA was extracted from mid-third instar or late third instar larvae (-18 or -4 hrs relative to puparium formation), newly-formed prepupae (0 hrs), or at two hour intervals after puparium formation until 12 hrs. Northern blots were probed to detect BR-C, E74A, E74B, E75A, DHR4, FTZ-F1, Sgs-4, Ddc, IMP-L1, L71-1, and Fbp1. Hybridization to detect rp49 mRNA was performed as a control for loading and transfer.