Skip to main content
. Author manuscript; available in PMC: 2010 Mar 25.
Published in final edited form as: Nat Med. 2009 Jan 18;15(2):206–210. doi: 10.1038/nm.1906

Figure 6. SIRT6 regulates TNF-α protein synthesis.

Figure 6

(A) The human cell line 293T was transiently transfected with plasmids encoding the indicated tagged forms of murine sirtuin members. Protein expression was determined by western blot using an anti-FLAG mAb. (B) Pools of 293T cells were transiently transfected with three constructs encoding respectively for human TNF-α, mouse IL-2 and a selected murine sirtuin as indicated. The results represent the relative content of human TNF-α compared to murine IL-2 released in the supernatant during 6 hours of culture. (C) The human cell line 293T was transiently transfected with plasmids encoding the indicated tagged forms of wt and mutant forms of selected murine sirtuin members. Protein expression was determined as in (A). (D) TNF-α translation efficiency of triple transfectants, as described in (B), was determined as detailed in the text (mean of two independent experiments). (D) Bone marrow derived dendritic cells from wt and SIRT6 KO mice were stimulated with 1 μg/ml CpG and TNF-α protein levels determined in the supernatant collected at the indicated timing.