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. Author manuscript; available in PMC: 2011 Mar 15.
Published in final edited form as: Dev Biol. 2010 Jan 11;339(2):429–438. doi: 10.1016/j.ydbio.2010.01.002

Figure 3. Lymph heart muscle but not lymphatic endothelium requires Hh signaling.

Figure 3

Cyclopamine treatment greatly reduces en1 expression in the somites (23 out of 24 embryos), while neural expression is relatively unaffected (B compared to A, arrow in A indicates lymph heart expression). At later stages, 12/101 staining shows that lymph heart musculature is missing in cyclopamine-treated tadpoles (D compared to C, arrow in C indicated lymph heart muscle). The presence of Prox1-positive lymphatic endothelial cells (arrowheads) is not affected by cyclopamine treatment at stage 39 (F compared to E) or stage 42 (H compared to G). (H) The loss of lymph heart musculature leads to a less compact distribution of Prox1-positive cells in the lymph heart (arrow).