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. Author manuscript; available in PMC: 2011 Mar 15.
Published in final edited form as: Dev Biol. 2010 Jan 11;339(2):429–438. doi: 10.1016/j.ydbio.2010.01.002

Figure 7. Engrailed-1 is required for lymph heart myogenesis.

Figure 7

Two non-overlapping MOs were designed to block en1 translation. Bilateral injection of En1 MO leads to loss of lymph heart beat and edema (A–C). Unilateral injection of En1 MO leads to specific loss of lymph heart musculature assayed by en1 expression (D–F, *p<0.05 χ2 test) and 12/101 staining (G–I, dorsal view, arrow points to lymph heart, asterisk indicates injected side). In controls, 100% of embryos have two 12/101-positive lymph hearts (N=43), while 5% of En1 morphants do (En1 MO1 N=42, En1 MO2 N=38). Lymph heart endothelium (prox1, J–L) is not affected by En1 knockdown compared to controls (p>0.05, χ2 test).