Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
In vitro stimulation of PBMCs. sCD14 levels in cell-culture supernatants were quantified in duplicates by ELISA. * p < 0.05 vs medium treatment. PBMCs (2 × 106) in RPMI medium were stimulated with PMA (10 ng/ml), fMLP (100 ng/ml) or the TLR ligands CpG-DNA (100 μg/ml), zymosan (50 μg/ml), PGN (1 μg/ml), Pam3CSK4 (1 μg/ml), flagellin (1 μg/ml) or LPS (100 ng/ml) for 1 hour (A) or 40 hours (B) at 37°C.
