Table 5.
APCI MS Data (Positive Ion) | |||||||
---|---|---|---|---|---|---|---|
Peak | Identification | Time (min) | M/QMIPa | Other Peaks | MS2 of M/QMIP | Plant Sourceb | Content in formulation (mg/kg) |
1 | Esculin | 6.4 | 341 (M+H)+ | 179 | 179 | FR | |
2 | Chlorogenic acid | 10.3 | 355 (M+H)+ | 163 | 163 | AS | |
3 | Aesculetin | 12.0 | 179 (M+H)+ | 134, 123, 109 | FR | 310 | |
4 | Paeoniflorin | 18.8 | 498 (M+H2O)+ | 301, 179 | 301, 179 | PL | |
5 | prim-O-Glucosylcimifugin | 23.8 | 469 (M+H)+ | 307 | SD | ||
6 | Magnoflorine | 32.2 | 342 (M)+ | 297, 265 | CC, PA | ||
7 | Liquiritin | 35.9 | 419 (M+H)+ | 307, 257 | GU | ||
8 | 5-O-Methylvisamminoside | 49.9 | 453 (M+H)+ | 291 | 290 | SD | |
9 | Hesperidin | 52.0 | 610 (M)+ | 465, 449, 303 | 463 | CR | 1460 |
10 | Columbamine | 60.2 | 338 (M)+ | 323, 294 | CC | ||
11 | Jatrorrhizine | 61.3 | 338 (M)+ | 323, 294 | CC | ||
12 | Epiberberine | 61.3 | 336 (M)+ | CC | |||
13 | Coptisine | 61.4 | 320 (M)+ | 304, 292 | CC | ||
14 | Palmatine | 66.5 | 352 (M)+ | 337, 308 | CC, PA | 420 | |
15 | Berberine | 67.3 | 336 (M)+ | 321, 292 | CC, PA | 1620 | |
16 | Glycyrrhizic acid | 76.2 | 823 (M+H)+ | 647, 471, 453, 406 | GU | 780 | |
17 | Schisandrin | 81.8 | 433 (M+H)+ | 415 | SC | 140 | |
18 | Honokiol | 93.3 | 266 (M)+ | 263 | MO | 63 | |
19 | Magnolol | 98.6 | 266 (M)+ | 261 | MO | 93 | |
20 | Schisandrin A | 107.7 | 417 (M+H)+ | 402, 347, 316 | SC |
Note: Peak number refers to the chromatographic fingerprint of the clinical preparation (Figure 4). Peaks 6, 10, 12, and13 were identified by LC-MS/MS2 analysis based on the literature values [17,18] and other peaks were identified by comparison of authentic chemicals. Jatrorrhizine and epiberberine (peaks 11, 12) were co-eluted with the same retention times.
a Molecular or quasi-molecular ion peak; b FR: Fraxinus rhynchophylla; AS: Artemisia scoparia; PL: Paeonia lactiflora; SD: Saposhinikovia divaricata; GU: Glycyrrhiza uralensis; CR: Citrus reticulata; CC: Coptis chinensis; PA: Phellodendron amurense; SC: Schisandra chinensis; MO: Magnolia officinalis.