Figure 1.

Detection of apoptosis in cells treated for 24 h with paclitaxel or pancratistatin. Flow cytometry with Annexin-V AlexaFluor 488 was used to detect apoptosis in Jurkat cells (A) and patient-obtained leukemic PBMCs (B) that were untreated, treated with 500 nM paclitaxel or treated with 1 μM pancratistatin for 24 h. The leukemic PBMCs were obtained from a chemo-näive female patient (65 y) diagnosed with AML -- M1. Flipping of phosphatidylserine from the inner to the outer leaflet of the plasma membrane, which binds to Annexin-V in the presence of calcium, is a characteristic feature of apoptosis. A minimum of 20 000 events were measured for each sample on a Beckman Coulter Cytomics FC500 flow cytometer.