Table 1.
Hippocampal concentrations of PKC isoforms in soluble and particulate subcellular fractions of young and aged rats
PKC isoform | Subcellular fraction | Young rats | Aged rats |
---|---|---|---|
γ* | Soluble | 9.90 ± 0.37 | 10.70 ± 0.28 |
Particulate | 8.08 ± 0.60 | 8.85 ± 0.37 | |
α | Soluble | 7.22 ± 0.30 | 7.10 ± 0.27 |
Particulate | 5.81 ± 0.44 | 5.44 ± 0.30 | |
β2 | Soluble | 5.68 ± 0.39 | 6.13 ± 0.34 |
Particulate | 4.66 ± 0.48 | 4.73 ± 0.38 |
Values represent the mean ± SEM of integrated optical density and area measures of individual samples immunostained for PKC isoforms and calibrated to standards in μg of total protein (see Fig. 1).
Aged rats had higher overall concentrations of PKCγ than young rats, but the age x subcellular fraction interaction was not significant.