Table 1. Cell recruitment after i.t. Klebsiella challenge in WT and mutant mice.
Cell count | WT/Un | WT/kp | TLR4 lps-d/kp | TLR9−/−/kp | TLR4 lps-d9−/−/kp |
Total cells | 1.2±0.2×106 | 24.6±2.2×106 | 24.2±1.3×106 | 25.5±1.3×106 | 24.2±2.5×106 |
PMN | 0.4±0.73×106 | 8.2±1.32×106 | 3.1±1.01×106* | 8.9±1.1×106 | 3.5±0.4×106* |
Monocytes/Macrophages | 0.8±0.4×106 | 15.2±2.06×106 | 21.2±2.06×106 | 17.7±2.03×106 | 21.5±3.03×106 |
Total cells | ND | 32.3±1.3×106 | 33.1±3.5×106 | 31.2±3.3×106 | 33.3±4.4×106 |
PMN | ND | 16.2±0.3×106 | 12.5±0.2×106 | 14.8±1.04×106 | 14.8±1.04×106 |
Monocytes/Macrophages | ND | 16.1±2.03×106 | 20.2±2.34×106 | 16.6±1.15×106 | 19.5±1.05×106 |
Leukocytes were harvested from lung 6 and 24 hrs post Klebsiella administration by collagenase lung digest, and cytospins performed. *p<0.05 as compared to WT mice. n = 5 in each group. Un = untreated, ND = not done.