Figure 1. Depletion of human large-GEFs using siRNA.

A: HeLa cells were transfected without siRNA (Mock), with non-targeting siRNA duplexes (Control), or with siRNA targeting indicated GEF (GBF1, BIG1, BIG2). Cell lysates were then immunoblotted for antibodies indicated on the left (Blot). Arrowhead points to the specific band for BIG1. B: Western-blots were quantified by densitometry and protein contents are expressed as percentage of mock-transfected cells. Results are presented as mean +/− s.e.m., n = 3–4, asterisk means p<0.05 as compared to cells transfected with a non-targeted siRNA (Control). Molecular-mass markers are shown in kDa in all figures.