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. 2010 Mar 25;5(3):e9898. doi: 10.1371/journal.pone.0009898

Figure 5. The fragmented Golgi induced by BIG1 suppression remains functional.

Figure 5

A: Cells were infected with adenovirus to express tsO45-G-YFP at 39.5°C. Cells were then shifted to 32°C to allow the trafficking of the viral protein for 0, 45, or 120 minutes as indicated. Trafficking of the viral protein is indistinguishable between control and BIG1 depleted cells. Note the fragmentation of the Golgi in BIG1 depleted cells confirming the efficacy of BIG1 suppression. B: The fragmented Golgi in BIG1-depleted cells is still populated with endogenous galactosyltransferase (GalT). Depleted HeLa cells were processed for immunofluorescence with anti-GalT (green in merge) and anti-giantin (red) antibodies. C: Scattered Golgi elements induced by BIG1-depletion retain their cis-trans polarity. HeLa cells transfected with siRNA duplexes as indicated were incubated with nocodazole (Nz) to depolymerize the microtubules, methanol-fixed, and processed for immunofluorescence with anti-TGN46 (green in merge) and anti-GM130 (red) antibodies.