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. Author manuscript; available in PMC: 2010 Nov 12.
Published in final edited form as: Vaccine. 2009 Sep 9;27(48):6678–6687. doi: 10.1016/j.vaccine.2009.08.093

Figure 1. Effects of numbers and conservation of amino acid substitutions on recognition of T-cell epitope variants in subjects with primary HIV-1 infection (A, B) and in HIV-1 vaccine recipients (C, D).

Figure 1

PBMC were stimulated with HIV-1 PTE peptide sets in a matrix format and tested for IFN-γ production using an ELISpot assay. Responses were examined to the Nef protein in subjects with primary infection and to Gag, Env and Pol in the vaccinees. Responses > 3-fold background and > 50 SFC/106 cells above background were scored positive. All responses were confirmed at the single peptide level. Shown is the frequency of recognition of epitope variants containing one, two and three amino acid substitutions; and conservative, semi-conservative and non-conservative substitutions.