Skip to main content
. Author manuscript; available in PMC: 2010 Nov 12.
Published in final edited form as: Vaccine. 2009 Sep 9;27(48):6678–6687. doi: 10.1016/j.vaccine.2009.08.093

Figure 2. IFN-γ and Granzyme B responses to optimal epitope variants in the Nef protein.

Figure 2

Figure 2

Standard IFN-γ and granzyme B ELISpot assays were performed by stimulating with the optimal epitopic peptides and variants at serial concentrations between 10,000 and 0.1 ng/ml. Shown here are PBMC responses from subjects 1188 (WF9 epitope variants) (A) and 1692 (RW8 epitope variants) (B). The SFC frequencies are plotted against the log10 peptide concentration. CON B sequence for each epitope is indicated on the top. Identity of autologous sequence to amino acid in the CON sequence is indicated by a dot.