. Author manuscript; available in PMC: 2011 Mar 9.

Published in final edited form as: Biochemistry. 2010 Mar 9;49(9):1975–1984. doi: 10.1021/bi901867s

Table 2.

Effects of TnC mutations on the Ca²⁺ binding properties of the reconstituted thin filaments in the absence and presence of myosin S1.

Protein

TF
Ca²⁺ K_d
(nM)

TF
Hill
coefficient

TF
Ca²⁺ K_off
(/s)

TF+S1
Ca²⁺ Kd
(nM)

TF+S1
Hill
coefficient

TF+S1
Ca²⁺ K_off
(/s)

{TnC}_{IAANS}^{T 53 C}

5027±97

1.45±0.03

102±1

1007±72

1.00±0.03

12.6±0.1

F 20 {QTnC}_{IAANS}^{T 53 C}

6803±604

1.38±0.02

221±12^*

8598±250^*

0.93±0.01

42±1^*

V 44 {QTnC}_{IAANS}^{T 53 C}

841±124^*

0.87±0.04^*

22.2±0.3^*

522±59^*

0.97±0.04

6.2±0.1^*

M 45 {QTnC}_{IAANS}^{T 53 C}

449±33^*

1.46±0.08

31.9±0.5^*

814±30

1.02±0.09

2.7±0.1^*

L 48 {QTnC}_{IAANS}^{T 53 C}

168±7^*

1.7±0.1

22.9±0.5^*

241±11^*

0.94±0.05

2.1±0.1^*

M 81 {QTnC}_{IAANS}^{T 53 C}

4468±129^*

1.02±0.05^*

199±7^*

1403±61^*

0.91±0.01^*

35.7±0.6^*

Values marked with * are significantly different from their respective control values (P<0.05).