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. 2010 Mar 26;5(3):e9910. doi: 10.1371/journal.pone.0009910

Figure 4. The effects of the PDK1-IFPC::IFPN-AKT1 complex on cell viability.

Figure 4

Five cell lines were used in cell viability assays including parental HeLa and four stable HeLa cell lines expressing IFPN-AKT1 alone, co-expressing IFPN-AKT1 and PDK1-IFPC, co-expressing IFPN-AKT1 and IFPC-ACTN4, or expressing PDK1-GFP alone, respectively. Cells were plated in 96-well plates in complete medium at 3000 cells/well. After 18–24 hours, cells were treated with drugs at different concentrations for 24 hours. Cell viability was measured by CellTiter Blue Cell Viability assay according to manufacturer's instructions. Data presented as survival rates normalized to non-treatment controls, respectively. Error bars were the standard deviations of triplicates of each treatment. Cell viability in each treatment was also plotted against the logarithm of drug concentration by fitting to the 3-parameter log(inhibitor) vs. response curve in GraphPad Prism version 5.0. IC50 was calculated by interpolating corresponding cell viability curve. (A) The PDK1-IFPC::IFPN-AKT1 complex promotes cell viability with LY294002 or PI103 treatment. (B) The PDK1-IFPC::IFPN-AKT1 complex promotes cell viability with GDC0941 or TGX286 treatment. (C) The PDK1-IFPC::IFPN-AKT1 complex promotes cell viability with Akti-1/2 but has no effect on cell viability with rapamycin treatment. (D) The PDK1-IFPC::IFPN-AKT1 complex promotes cell viability with paclitaxel treatment and combined treatment with LY294002 and paclitaxel.