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. 2010 Mar 26;5(3):e9909. doi: 10.1371/journal.pone.0009909

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Excoffon et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Panel A shows co-localization (yellow) of hCAR^Ex8 (red) and PSD-95-GFP (green). In contrast, in panel B, hCAR^Ex8-PDZ does not co-localize at the junctions of cells. hCAR^Ex8-PDZ localizes to the junctions between cells whereas PSD-95-GFP fluorescence remains diffuse. Panel C shows immunoprecipitation of PSD-95-GFP with the hCAR specific extracellular domain monoclonal antibody RmcB, GFP antibody, but not a control antibody (MopC). Panels D and E shows the lack of co-localization and immunoprecipitation between hCAR^Ex8 (junctional) and PICK1-GFP (perinuclear). Confocal microscopy (60x oil immersion).