Figure 4. Co-expression of hCAR^Ex8 and MAGI-1b-GFP results in the loss of hCAR^Ex8.

In contrast to the co-localization of hCAR^Ex7 (A, red) and MAGI-1b-GFP (B, green) as shown in panel C (yellow), co-expression of hCAR^Ex8 (D, G, red) and MAGI-1b-GFP (E, H, green) results in decreased levels of hCAR^Ex8 (F) unless MAGI-1b-GFP is absent from the cell (I). Co-expression of hCAR^Ex8 (J, red) with GFP (K, green) results in abundant hCAR^Ex8 expression at the junctions of the cells and diffuse GFP expression (L). Confocal microscopy (60x oil immersion).