Figure 5. Co-expression of hCAR^Ex8 with MAGI-1b-GFP results in less immunofluorescence, protein, and adenovirus-mediated gene transfer.

COS-7 cells were transfected with 0 to 30 µg of hCAR^Ex8 +/− 15 µg MAGI-1b-GFP and evaluated for hCAR^Ex8 specific immunofluorescence (A) or CAR^Ex8 specific protein by Western blot (B). In the presence of MAGI-1b-GFP, the hCAR^Ex8 expression curve is shifted to the right suggesting a loss of hCAR^Ex8 protein. Panel C shows CHO cells transfected with varying amounts of hCAR^Ex8 and MAGI-1b-GFP, and evaluated for Ad-β-galactosidase gene transfer. Co-expression of MAGI-1b-GFP resulted in a decrease of adenovirus-mediated gene transfer in a dose response relationship. *p<0.03.