Figure 8. The interaction between the hCAR PDZ binding domain and PICK1 depends on both the PDZ binding domain and the upstream isoform specific amino acids.

The PDZ binding domain of hCAR^Ex7 and hCAR^Ex8 were swapped by PCR as shown in Figure 7, panel A. The localization was determined in transfected COS-7 cells. hCAR^Ex7 (A) transfected with PICK1-GFP (B) results in co-localization at the junctions (C, yellow). hCAR^Ex8 (D) transfected with PICK1-GFP (E) results in no co-localization at the junctions (F). hCAR^Ex7/8 (G) transfected with PICK1-GFP (H) results some co-localization at the junctions (I, yellow). hCAR^Ex8/7 (J) transfected with PICK1-GFP (K) results in no co-localization at the junctions (L). Confocal microscopy (60x oil immersion).