Table 2. Effect of caffeine on lens dulcitol.

Group	Dulcitol (µmoles/g wet wt.)
Galactose	17.2±2.5
Galactose + Caffeine	19.6±6

Rats weighing 40–45 g were fed a 24% galactose diet without or with 1% caffeine. There were then euthanized, lenses dissected out and as described, transferred on a Millipore grid and photographed by trans-illumination using 50 W daylight tube. Subsequently they were used to prepare a protein free filtrate by homogenization with Nelson-Semoygi reagent and centrifugation. Dulcitol was determined as described in the text. Values are expressed as mean±s.d., n=6 (number of animals).