Table 2. Primer sequences for the amplification of the coding region of DFNB31.
| Primer name | Sequence (5′-3′) | Annealing temperature |
|---|---|---|
| Exon 1 |
F: CAGCAGCCAACTCTTGTGTC |
55 °C |
| R: CCAGAAAGGCCAAGTGATTC | ||
| Exon 2 |
F: ACTCCCCAAATCCAAGTTCC |
58 °C |
| R: CAGAACCAGCCTCTTCTTGC | ||
| Exon 3 |
F: CTCCTTGCCAGTCGGATAAG |
55 °C |
| R: GAGTGCTGATTGCTCTGCTG | ||
| Exon 4 |
F: ATAAGGGGACCCTTGGAATG |
55 °C |
| R: TCCCCACTTTTTGGATGAAG | ||
| Exon 5 |
F: GTCCGGAGTTTCCTTTACCC |
55 °C |
| R: TGGTCTGCTCTGTTCATTGC | ||
| Exon 6 |
F: TGGCAATGAACAGAGCAGAC |
58 °C |
| R: GGAGGGCTTGTGAAGATGAC | ||
| Exon 7 |
F: GACAGGGAAGCAGGAGTGAG |
58 °C |
| R: GATTCGAACTCAGGCTGGTC | ||
| Exon 8 |
F: CAGCATCTCTGGCAGTTCAG |
58 °C |
| R: GGCTGTCATGGAGAGGAGAG | ||
| Exon 9 |
F: GTGACAAGCTCTGGCTGATG |
58 °C |
| R: TTCAAACTGGGGTCTCCAAC | ||
| Exon 10 |
F: GGTCTGGTTGAAAGGACAGG |
58 °C |
| R: GGCCTCCAGATTCCTAATCC | ||
| Exon 11 |
F: GAGGCTGAGATTGGTCTTGG |
55 °C |
| R: CCTAGGTCTGCCCTTGAGTG | ||
| Exon 12 | F: CCCTTTCTCAGCATCTCCAG |
58 °C |
| R: GTCTGCCTTGTCCTGCTCTC |
Amplification conditions were 95 °C 5 min followed by 35 cycles of 30 s at 95 °C, 30 s at an annealing temperature specific for each exon (column 3) and 30 s at 72 °C.