Table 1.
Amino acid sequences of apamin, PMI, and stingins and their dissociation equilibrium constants for p53-binding domains of MDM2 and MDMX. Each Kd value is the mean of three independent measurements (N.B. = no binding).
| Name | Sequence | Kd (nM) | |
|---|---|---|---|
| MDM2 | MDMX | ||
| Apamin |  |
N.B. | N.B. |
| PMI | 3.2 ± 1.1 | 8.5 ± 1.7 | |
| Stingin-1 | CNCKAPETFLCYWRCLQH | 25.1 ± 5.1 | 11.4 ± 2.3 |
| Stingin-2 | CNCKAPETFLCYWRCLQ | 35.2 ± 3.7 | 18.0 ± 2.3 |
| Stingin-3 | CNCKAPETFLCYWRCL | 57.5 ± 7.2 | 16.0 ± 4.5 |
| Stingin-4 | CNCKAPETAFCAYWCQLH | 83.2 ± 8.4 | 252 ± 23 |
| Stingin-5 | CNCKAPETAFCAYWCQL | 17.7 ± 4.0 | 93.4 ± 9.2 |