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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Biomed Mater Res A. 2010 May;93(2):673–686. doi: 10.1002/jbm.a.32485

Figure 4. Schemes for making F108-collagen I double patterns.

Figure 4

A. There are 4 possible schemes of application, each involving a patterning step of one material and a flooding step of the other. Each application scheme produces a region coated with a single material (either collagen or F108) and another region coated with both materials (collagen on F108 or F108 on collagen). These four candidate surfaces represent potential adhesive and anti-adhesive regions to cause cell patterning. B. The adhesion of SH-EP cells was evaluated on the four unpatterned candidate surfaces produced from the application schemes. Any candidate surface coated with collagen was highly adhesive; only F108 alone reduced cell adhesion. C. The adhesion produced by each candidate surface was calculated as a fraction of the cell adhesion on collagen, and then percent reduction in adhesion between the surfaces in each scheme was calculated. Schemes 1 and 2, in which collagen does not contact F108 in the application process, produced significant reductions in adhesion between the more and less adhesive candidate surfaces. *p<0.05, ***p < 0.001.