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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Biomed Mater Res A. 2010 May;93(2):673–686. doi: 10.1002/jbm.a.32485

Figure 6. NBL cells comply to collagen stripes and avoid stripes of F108.

Figure 6

Substrates were constructed by patterning FITC-labeled collagen (green stripes) in 60 mm tissue culture plates, followed by flooding with F108 (B-D). SH-EP cells fail to pattern, adhering to both collagen and tissue culture plastic when no F108 was applied (A), but localized on the patterned collagen when F108 was flooded (B). SH-SY5Y (C) and IMR-32 (D) cells also localized on the collagen/F108 stripes and avoided F108 regions. Cells were stained with phalloidin (red) and bisbenzimide (blue). T = tissue culture plastic, C = Collagen I, F = F108. Scale bar = 50μm.