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. Author manuscript; available in PMC: 2011 Mar 23.
Published in final edited form as: Curr Biol. 2010 Mar 11;20(6):538–543. doi: 10.1016/j.cub.2010.01.049

Figure 3. Cargo Trafficking Phenotypes are Distinct in Cells with Disrupted ADE, CDE, or DDE.

Figure 3

Notch staining (green) 5 hrs post-chase from live trafficking experiment. Surface-labeled Notch, which is degraded at this time point in WT discs (A), is prominently trapped in surface puncta in AP-2α with a few internal puncta also visible (B), cortically and in internal puncta in Chc (C), and in a diffuse cortical location in shi (D) mutant eye discs. Internally-trapped Notch (green) in Chc mutants colocalizes with the endosomal protein Hrs (red) (E). Dashed lines in A-D represent cell cortex as determined by F-actin staining. In B-E arrows indicate Notch localized at or near the cell surface, arrowheads indicate internalized Notch. Scale bar: 10μm.

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