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. Author manuscript; available in PMC: 2010 Sep 25.
Published in final edited form as: Nature. 2010 Mar 17;464(7288):597–600. doi: 10.1038/nature08848

Figure 2. dTrpA1 functions in chemosensors.

Figure 2

a–c, dTRPA1 expression. Arrows denote cell bodies, arrowheads distal neurites. Cuticle autofluorescence visible. (b), LSO sensilla numbered. apn: accessory pharyngeal nerve. d. dTrpA1 mutants lack dTRPA1 staining. e. eso, esophagus. f–i, Gr66a-Gal4 and dTRPA1 co-expression in LSO. (f) Nuclear and (g,h) membrane GFPs expressed using Gr66a-Gal4. j–l, PER to 350 mM sucrose containing 10mM NMM. (j, k) Ingestion permitted. l, tarsal contact only. j, dTRPA1 knockdown. k, dTRPA1 rescue. l, dTRPA1 gain-of-function. *: α=0.05, **: α=0.01, differ from Gal4 and UAS controls, Tukey HSD. j,k, n=3 groups of 7–8 flies, l, n≥10 flies.