Skip to main content
. Author manuscript; available in PMC: 2010 Sep 25.
Published in final edited form as: Nature. 2010 Mar 17;464(7288):597–600. doi: 10.1038/nature08848

Figure 4. TRPA phylogeny.

Figure 4

a, Conservation of residues implicated in electrophile detection. choannoflag, choannoflagellate. b, TRPA1-wt (wild type) and dTRPA1-2C channels in Xenopus oocytes. 60 sec pulses of AITC (0.1, 0.5, and 1.0 mM) were applied with 25 sec intervals. c. +60 mV currents normalized to channel's response to 1.0 mM AITC. *p<0.05, **p<0.001, unpaired t-test. d, Bayesian consensus phylogeny for TRPAs. Internal branches labeled by posterior probability (<0.5 branches collapsed). Red dot denotes ancestor of TRPA1 clade. e, Cladogram showing TRPA complements, including numbers of channels. Red dot denotes bilaterian ancestor. f, PAML residue identity estimates for ancestral TRPA1.