Figure 6.

The Expression Levels of the Selected Genes in Mature Leaves of the Analyzed Tobacco Lines.

In all real time RT–PCR (qRT–PCR) reactions, both Tac9 and UBQ2 served as references, except for the qRT–PCR shown in (A), in which only Tac9 was used as a reference gene. nS, optimal conditions; –S, 2 d of S-deficit. Each experiment was performed at least two times in triplicate using two independently isolated RNA templates, with similar results. The presented data are from one representative experiment performed in triplicate with SD indicated.