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SKAP1+/+ and SKAP1−/− activated T-cells migrate at the same speed and distance in response to SDF1. T-cells were plates in one well in a flow-chamber plate and SDF-1 was added to another well. Time lapse was used to record cell movement every 5 s for 120 cycles. Representative images at the adjusted time points (A) and motility (B) and the distance of cell movement (C) were analysed by Velocity software.
