Fig. 7.

Co-localization of RPTPJ, RPTPκ, RPTPγ, RPTPε, RPTPRR and RPTPσ proteins in nestin-positive neural progenitor cells in the E13 mouse superiors colliculus. Sagittal sections through the embryonic E13 superior colliculus were double-labeled with antibodies against RPTPs and the neural progenitor marker nestin. Arrowheads indicate examples of double-labeled cells. Note that RPTPγ, RPTPε, RPTPRR and RPTPσ epitopes partially co-localize with nestin-positive progenitors. In contrast, RPTPJ was not co-localized with nestin-positive cells. Since nestin produces strictly radial staining whereas RPTPJ, RPTPγ, RPTPε, RPTPRR and RPTPσ antibodies label mainly nuclei/perikarya of collicular cells, both markers reveal a faint overlapping expression pattern (merge). As shown for RC-2 immunostainings, RPTPκ immunoreactivity directly overlaps with nestin-intermediate filament staining (merge). In comparison to the RC-2/RPTPκ overlap, the correspondence of nestin and RPTPκ appears less extensive. IZ intermediate zone, VZ/SVZ ventricular zone/subventricular zone. Inserts: Higher magnification of immunopositive cells (merge figures represent co-immunoreactive cells). Scale bar 50 μm