Fig. 8.

Co-localization of PTP1C, PTP1D, PTP-MEG2 and PTP-PEST proteins in RC-2-positive radial glia cells in the E13 mouse superior colliculus. Sagittal sections through the embryonic E13 superior colliculus were double-labeled with antibodies against intracellular PTPs and the radial glia marker RC-2. Arrowheads indicate examples of double-labeled cells. PTP1D, PTP-MEG2 and PTP-PEST epitopes partially co-localize with RC-2-positive radial glia cells. In contrast, PTP1C immunoreactivity shows a less pronounced co-localization with RC-2 positive cells in the ventricular zone and does not co-localize with minor RC-2-positive cells in the intermediate zone. Note that the antibodies directed at intracellular PTPs label mainly nuclei/perikarya of collicular cells; both markers reveal an incomplete overlapping expression pattern (merge). IZ intermediate zone, VZ/SVZ ventricular zone/subventricular zone. Inserts: Higher magnification of immunopositive cells (merge figures represent co-immunoreactive cells). Scale bar 50 μm