Table 2.
HIV-1 Specific IFNγ Responses Measured by ELISPOT
| Peptide (Pool #) # of peptides (amino acids) | Baseline Frequencies* | Change from Baseline** Week 6 (Post MVA) n=19 | Change from Baseline** Week 26 (Post FP) n=10 |
|---|---|---|---|
| Gag (1) | 188 (70, 500) | 92 (−57, 258) | 281 (−103, 494) |
| 61 (1–255) | p = 0.258 | p = 0.105 | |
| Gag (2) | 86 (34, 249) | 64 (−7, 131) | 60 (9, 455) |
| 62 (245–500) | p = 0.130 | p = 0.020 | |
| Pol (1) | 39 (2, 296) | 16 (−2, 126) | 36 (−51, 366) |
| 83 (1–343) | p = 0.167 | p = 0.322 | |
| Pol (2) | 24 (14, 256) | 43 (18, 152) | 80 (37, 146) |
| 83 (333–675) | p = 0.010 | p = 0.004 | |
| Pol (3) | 41 (20, 79) | 19 (−9, 104) | 39 (15, 179) |
| 83 (665–1003) | p = 0.031 | p = 0.008 | |
| Env (1) | 33 (3, 75) | 0 (−35, 38) | −3 (−8, 12) |
| 71 (1–295) | p = 0.644 | p = 0.910 | |
| Env (2) | 7 (0, 82) | 2 (−12, 56) | 0 (−5, 6) |
| 71 (285–579) | p = 0.495 | p = 0.938 | |
| Env (3) | 87 (18, 186) | −8 (−42, 56) | −3 (−83, 7) |
| 70 (569–857) | p = 0.899 | p = 0.820 | |
| Tat | 0 (0, 21) | 8 (0, 35) | 3 (0, 33) |
| 23 (1–101) | p = 0.019 | p = 0.078 | |
| Rev | 1 (0, 20) | 5 (−4, 63) | 25 (3, 57) |
| 27 (1–117) | p = 0.102 | p = 0.014 | |
| Nef | 196 (152, 405) | 54 (−56, 521) | 29 (−319, 1050) |
| 49 (1–206) | p = 0.123 | p = 0.193 | |
*
SFC/million PBMC; Median frequencies (interquartile range); values background subtracted.
**
SFC/million PBMC; Median change from baseline, (interquartile range); p-values of Wilcoxon sign rank test comparisons of baseline with post-immunization responses, shaded cells indicate p <0.05.